王士安-中国科学院大学-UCAS
[中文]
[English]
研究领域
招生信息
教育背景
工作经历
教授课程
专利与奖励
出版信息
科研活动
指导学生
基本信息
王士安 男 博导 中国科学院青岛生物能源与过程研究所电子邮件： wangsa@qibebt.ac.cn通信地址： 山东省青岛市松岭路189号邮政编码： 266101近5年，针对非传统酵母遗传操作技术不完善的现状，优化解脂耶氏酵母和红法夫酵母研究平台，建立了遗传标记去除、CRISPR-Cas9基因编辑等技术；解脂耶氏酵母合成神经酸和红法夫酵母合成虾青素两项技术实现了企业合作转化。主持国家自然科学基金委、科技部和中科院项目或课题10余项。以第一作者或通讯作者在Biotechnology for Biofuels、Carbohydrate Polymers、Appl Environ Microbiol等SCI期刊发表论文20余篇；申请发明专利10项，PCT专利1项，已获授权专利2项。任Bioresources and Bioprocessing杂志青年编委（2020.7.1-2022.6.30）。合作培养博士生3名，硕士生5名。
研究领域
微生物合成生物学、代谢工程酵母菌生物技术酵母菌蛋白表达生物能源、医药
招生信息
微生物学、生物化学与分子生物学、生物与医药
招生专业
071010-生物化学与分子生物学071005-微生物学086000-生物与医药
招生方向
合成生物学，代谢工程微生物生理代谢蛋白表达
教育背景
2003-09--2008-06 中科院微生物研究所 博士1999-09--2003-06 陕西师范大学 学士
学历
研究生
学位
博士
工作经历
2017-01--今 中科院青岛生物能源与过程研究所 研究员 2013-01--2016.12 中科院青岛生物能源与过程研究所 副研究员2013-08--2014-08 美国麻省理工学院 访问学2008-07--2012-12 中科院青岛生物能源与过程研究所 助理研究员
教授课程
分子生物学前沿课程
专利与奖励
专利成果
[1] 孟慧敏, 王士安, 李家欣, 李福利. 生产神经酸的重组酵母菌株及其应用.&nbspCN:&nbspCN110358692B,&nbsp2021-07-27.[2] 王士安, 张宁, 李福利. 一种提高红发夫酵母虾青素产量的方法.&nbspCN:&nbspCN112430637A,&nbsp2021-03-02.[3] 王士安, 李家欣, 李福利. 一种提高解脂耶氏酵母油脂产量的方法.&nbspCN:&nbspCN112391401A,&nbsp2021-02-23.[4] 王士安, 赵晨, 李福利. 一种圆红冬孢酵母RNA启动子及其应用.&nbspCN:&nbspCN112143728A,&nbsp2020-12-29.[5] 王士安, 张宁, 李福利. 一种启动红发夫酵母RNA表达的启动子及其应用.&nbspCN:&nbspCN112143729A,&nbsp2020-12-29.[6] 王士安, 李福利. 一种高产虾青素红酵母工程菌及其构建方法.&nbspCN:&nbspCN106318878B,&nbsp2019-12-20.[7] 王达, 王士安, 李福利. 一种发酵菊糖产低聚果糖的酿酒酵母及其构建方法与应用.&nbsp中国:&nbspCN107460137A,&nbsp2017-12-12.[8] 王士安, 袁博, 李福利. 一种乙酸耐性乙醇生产酿酒酵母菌株及菌株筛选方法.&nbsp中国:&nbspCN102146345A,&nbsp2011-08-10.
出版信息
发表论文
[1] Jixuan Hong, Ziyue Meng, Zixi Zhang, Shi'an Wang. Comprehensive analysis of CRISPR-Cas9 editing outcomes in yeast Xanthophyllomyces dendrorhous.. The CRISPR Journal[J]. 2022, [2] Wang Shian. Disrupting a phospholipase A2 gene increasing lipid accumulation in the oleaginous yeast Yarrowia lipolytica. J Appl Microbiol. 2020, [3] 赵晨, 孟慧敏, 李家欣, 张培玉, 李福利, 王士安. 解脂耶罗维亚酵母工程菌合成超长链脂肪酸及温度的影响. 微生物学通报[J]. 2020, 47(1):&nbsp13-23, http://lib.cqvip.com/Qikan/Article/Detail?id=7101120416.[4] Zhang, Ning, Li, Jiaxin, Li, Fuli, Wang, Shian. Selectable marker recycling in the nonconventional yeast Xanthophyllomyces dendrorhous by transient expression of Cre on a genetically unstable vector. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2019, 103(2):&nbsp963-971, https://www.webofscience.com/wos/woscc/full-record/WOS:000457830000034.[5] Zhang, Ning, Fan, Yuxuan, Li, Chen, Wang, Qiming, Leksawasdi, Noppol, Li, Fuli, Wang, Shian. Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2018, 102(9):&nbsp4183-4191, http://www.irgrid.ac.cn/handle/1471x/1755518.[6] Wang, Da, Li, FuLi, Wang, ShiAn. Engineering a natural Saccharomyces cerevisiae strain for ethanol production from inulin by consolidated bioprocessing. BIOTECHNOLOGY FOR BIOFUELS[J]. 2016, 9: http://ir.qibebt.ac.cn/handle/337004/8309.[7] Alimadadi, Nayyereh, Soudi, Mohammad Reza, Wang, ShiAn, Wang, QiMing, Talebpour, Zahra, Bai, FengYan. Starmerella orientalis f.a., sp nov., an ascomycetous yeast species isolated from flowers. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY[J]. 2016, 66: 1476-1481, http://dx.doi.org/10.1099/ijsem.0.000905.[8] Wang, Da, Li, FuLi, Wang, ShiAn. A one-step bioprocess for production of high-content fructo-oligosaccharides from inulin by yeast. CARBOHYDRATE POLYMERS[J]. 2016, 151: 1220-1226, http://dx.doi.org/10.1016/j.carbpol.2016.06.059.[9] Hu, Guangrong, Ji, Shiqi, Yu, Yanchong, Wang, Shian, Zhou, Gongke, Li, Fuli, Mukherjee, J. Organisms for Biofuel Production: Natural Bioresources and Methodologies for Improving Their Biosynthetic Potentials. BIOTECHNOLOGICAL APPLICATIONS OF BIODIVERSITY[J]. 2015, 147: 185-224, http://dx.doi.org/10.1007/10_2013_245.[10] Zheng, YanLin, Wang, ShiAn. Stress Tolerance Variations in Saccharomyces cerevisiae Strains from Diverse Ecological Sources and Geographical Locations. PLOS ONE[J]. 2015, 10(8):&nbsphttp://ir.qibebt.ac.cn/handle/337004/6509.[11] 田德雨, 王士安, 王立昊, 王家林, 李福利. 超长链单不饱和脂肪酸的生物合成和代谢工程. 生物技术通报[J]. 2015, 31(12):&nbsp1-, http://ir.qibebt.ac.cn/handle/337004/8675.[12] Wang, ShiAn, Li, FuLi. Invertase SUC2 Is the Key Hydrolase for Inulin Degradation in Saccharomyces cerevisiae. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2013, 79(1):&nbsp403-406, http://dx.doi.org/10.1128/AEM.02658-12.[13] []. Wang, S. A., Li, F. L. (2013). Invertase SUC2 is the key hydrolase for inulin degradation in Saccharomyces cerevisiae. Appl Environ Microbiol, 79(1), 403-406.. APPL ENVIRON MICROB[J]. 2013, http://ir.qibebt.ac.cn/handle/337004/8828.[14] []. Yuan, B., Wang, S. A., Li, F. L. (2013). Improved ethanol fermentation by heterologous endoinulinase and inherent invertase from inulin by Saccharomyces cerevisiae. Bioresour Technol, 139, 402-405. doi: 10.1016/j.biortech.2013.04.076. BIORESOURCE TECHNOL[J]. 2013, http://ir.qibebt.ac.cn/handle/337004/8858.[15] Yuan, Bo, Wang, ShiAn, Li, FuLi. Expression of exoinulinase genes in Saccharomyces cerevisiae to improve ethanol production from inulin sources. BIOTECHNOLOGY LETTERS[J]. 2013, 35(10):&nbsp1589-1592, http://www.irgrid.ac.cn/handle/1471x/830089.[16] Yuan, Bo, Wang, ShiAn, Li, FuLi. Improved ethanol fermentation by heterologous endoinulinase and inherent invertase from inulin by Saccharomyces cerevisiae. BIORESOURCE TECHNOLOGY[J]. 2013, 139: 402-405, http://dx.doi.org/10.1016/j.biortech.2013.04.076.[17] Yuan, B., Hu, N., Sun, J., Wang, S. A., Li, F. L.. Purification and characterization of a novel extracellular inulinase from a new yeast species Candida kutaonensis sp. nov. KRF1(T). APPL MICROBIOL BIOT[J]. 2012, 96(6):&nbsp1517-1526, http://ir.qibebt.ac.cn/handle/337004/8925.[18] Wang, QiMing, Liu, WanQiu, Liti, Gianni, Wang, ShiAn, Bai, FengYan. Surprisingly diverged populations of Saccharomyces cerevisiae in natural environments remote from human activity. MOLECULAR ECOLOGY[J]. 2012, 21(22):&nbsp5404-5417, http://dx.doi.org/10.1111/j.1365-294X.2012.05732.x.[19] Ji, Shiqi, Wang, Shian, Tan, Yang, Chen, Xiaohua, Schwarz, Wolfgang, Li, Fuli. An untapped bacterial cellulolytic community enriched from coastal marine sediment under anaerobic and thermophilic conditions. FEMS MICROBIOLOGY LETTERS[J]. 2012, 335(1):&nbsp39-46, http://www.irgrid.ac.cn/handle/1471x/519118.[20] Yuan, Bo, Hu, Nan, Sun, Juan, Wang, ShiAn, Li, FuLi. Purification and characterization of a novel extracellular inulinase from a new yeast species Candida kutaonensis sp nov KRF1(T). APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2012, 96(6):&nbsp1517-1526, http://ir.qibebt.ac.cn/handle/337004/6390.[21] Hu, Nan, Yuan, Bo, Sun, Juan, Wang, ShiAn, Li, FuLi. Thermotolerant Kluyveromyces marxianus and Saccharomyces cerevisiae strains representing potentials for bioethanol production from Jerusalem artichoke by consolidated bioprocessing. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2012, 95(5):&nbsp1359-1368, http://dx.doi.org/10.1007/s00253-012-4240-8.[22] 何茹, 刘君寒, 王士安, 李福利. 小球藻鉴定基因的筛选及高产油微藻的分子鉴定. 安徽农业科学[J]. 2011, 39(25):&nbsp15230-15232, http://lib.cqvip.com/Qikan/Article/Detail?id=39253628.[23] Wang, ShiAn, Li, FuLi, Bai, FengYan. Candida laoshanensis sp nov and Candida qingdaonensis sp nov., anamorphic, ascomycetous yeast species isolated from decayed wood. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY[J]. 2010, 60: 1697-1701, http://124.16.144.217/handle/311023/2147.[24] Wang Shian. Candida laoshanensis and Candida qingdaonensis, novel anamorphic, ascomycetous yeast species isolated from decayed wood. Int J Syst Evol Microbiol. 2010, [25] ShiAn Wang, FengYan Bai. Lindnera wuzhiensis sp. nov., a novel ascomycetous yeast species. 2010, http://kns.cnki.net/KCMS/detail/detail.aspx?QueryID=0&CurRec=1&recid=&FileName=SJJS07F4D4EDC6718EA842DA12516B575755&DbName=SJJSLAST&DbCode=SJJS&yx=&pr=&URLID=&bsm=.[26] Wang, ShiAn, Bai, FengYan. Lindnera wuzhiensis sp nov., a novel ascomycetous yeast species. JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY[J]. 2010, 56(5):&nbsp409-412, http://ir.qibebt.ac.cn/handle/337004/6568.[27] 王士安, 胡南, 张坤迪, 李福利. 酿酒酵母麦芽汁糖代谢与啤酒发酵度研究. 酿酒科技. 2010, 44-47, http://lib.cqvip.com/Qikan/Article/Detail?id=35441315.[28] ShiAn Wang, JianHua Jia, FengYan Bai. Candida alocasiicola sp. nov., Candida hainanensis sp. nov., Candida heveicola sp. nov. and Candida musiphila sp. nov., novel anamorphic, ascomycetous yeast species isolated from plants. Antonie van Leeuwenhoek,. 2008, 94(2):&nbsp[29] Wang, ShiAn, Bai, FengYan. Saccharomyces arboricolus sp nov., a yeast species from tree bark. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY[J]. 2008, 58: 510-514, http://124.16.144.217/handle/311023/2094.[30] Wang, QiMing, Li, Juan, Wang, ShiAn, Bai, FengYan. Rapid differentiation of phenotypically similar yeast species by single-strand conformation polymorphism analysis of ribosomal DNA. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2008, 74(9):&nbsp2604-2611, http://www.corc.org.cn/handle/1471x/2386498.
科研活动
科研项目
（ 1 ）&nbsp中科院创新促进会, 主持, 部委级, 2013-01--2016-12（ 2 ）&nbsp红发夫酵母油脂积累对虾青素合成的正负调控及机理研究, 主持, 国家级, 2017-01--2020-12（ 3 ）&nbsp粘红酵母基于挥发性脂肪酸的油脂合成机理研究, 参与, 国家级, 2017-01--2020-12（ 4 ）&nbsp颗粒型甲烷单加氧酶在酿酒酵母的工程化组装, 主持, 市地级, 2018-05--2019-05（ 5 ）&nbsp酵母发酵生产神经酸的中试及示范, 主持, 部委级, 2019-01--2020-06（ 6 ）&nbsp生物发酵法生产神经酸技术, 主持, 院级, 2018-05--2021-05（ 7 ）&nbsp微藻高效供碳和高值化产品关键技术开发与示范, 主持, 国家级, 2019-08--2022-07（ 8 ）&nbsp基于生物质的微生物油脂合成及航空煤油炼制过程的基础研究, 主持, 国家级, 2018-08--2021-07（ 9 ）&nbsp高产虾青素红发夫酵母菌种及工艺开发, 主持, 院级, 2020-07--2022-12
参与会议
（1）红发夫酵母基因编辑技术及萜烯合成调控 中国菌物学会2019年学术年会 2019-08-04（2）Genetic and Metabolic Engineering of the Nonconventional Yeast X. dendrorhous for Production of Terpene Compounds 2019-07-01（3）Development of unconventional yeast systems for biosynthesis 2018-02-01（4）酵母菌合成燃料乙醇及高值化合物 2017-05-02（5）酿酒酵母菊糖代谢及菊芋乙醇代谢工程 2015年中国微生物学会学术年 2015-10-25（6）Development of Acetic-Acid Tolerant Saccharomyces Cerevisiae Strains 王士安 2010-02-02
指导学生
已指导学生范雨萱 硕士研究生 085238-生物工程
2013 中国科学院大学，网络信息中心.